Abstracts DGMKG-DGNC-DGPRÄC-DGKCH

2022 Innovative Surgical Science  
In order to help individualize therapy principles for oral squamous cell carcinoma (OSCC), analysis of different biomarkers for metastasis, tumor recurrence, resistance mechanism and outcome seems mandatory. Slow Off-rate Modified aptamers as modified DNA aptamers as used in the SOMAScan® (Somalogic, Boulder, USA) with high affinity and high specificity to the respective proteins, may overcome current limitation in proteome analysis. So far, this technology was not evaluated for OSCC.
more » ... aim of this feasibility study is to firstly assess this method to identify distinct proteomic changes in the peripheral blood of patients with OSCC in comparison to healthy control. Materials and methods: From pre-and postresection 2ml sera as excess material of a well-defined study cohort (OSCC (n=6) vs. an age-matched healthy control group receiving dental surgery (n=4)) was obtained after informed consent. SOMAscan® analysis (Somalogic, Boulder, USA) was performed. Briefly, novel modified DNA aptamers (SOMAmers) were conducted. Following immobilization and denaturation, SOMAmers were released from associated proteins and hybridized to custom micro-array chips, containing complementary samples to each individual SOMAmer and measured via Cy3 fluorescence intensity. Results: The bio-informatic analysis revealed a total of 77 significantly differently expressed proteins (each p<0.05) that could discriminate between tumor and healthy control with a 100% accuracy in hierarchical clustering. Furthermore, 76 proteins were dectected that were significantly (each p<0.05) differently expressed between pre-and postresection sera samples. In the subsequent canonical pathwayanalysis, complementfactors, High-Mobility-Group-Pathway-1 (HMGB-1 proteins), apoptosis signaling, and transcription factors were statistically significant regulatory altered between the pre-and postresection liquid biopsy (p<0.05). Especially MYC and TWIST pathways showed significantly differences in up-and downstream of different regulator proteins. Conclusion: The quantitative proteome analysis with modified aptamers is feasible and may be used to develop precise serum marker to predict tumor occurrence, progression and recurrence in OSCC.
doi:10.1515/iss-2022-2005 fatcat:rcrdth7nufgxpo433ogjq7howe