Radioimmunoassay of Porcine Alpha Fetoprotein

R. T. Stone, R. R. Maurer
1979 Biology of Reproduction  
The development of a radioimmunoassay for porcine alpha fetoprotein (AFP) is described. Antibodies to AFP were produced in rabbits by injecting polyacrylamide gel slices containing AFP. Alpha fetoprotein was purified from amniotic fluid by ion exchange chromatography and preparative polyacrylamide gel electrophoresis. Alpha fetoprotein prepared in this manner was estimated to be >95% pure and was suitable as a reference standard for a radioimmunoassay. The radioimmunoassay was sensitive over a
more » ... ange of 0.6-34 ng. Levels of AFP in chorionic and amniotic fluids from 40-day-old fetal pigs from 8 sows were 171 ± 24 and 256 ± 20 pg/mI, respectively. Maternal serum levels were 16 ± 2 ng/ml at estrus and did not show a significant increase until Day 35 (23 ± 2 ng/ml) of gestation. From Day 50 to Day 110 of gestation, maternal serum levels of AFP were highest at Day 70 (58 ± 14 ng/ml); the values at this stage of gestation were quite variable, ranging from 21 to 180 ng/ml. Serum levels of AFP in boars were 16 ± 2 ng/ml and this AFP was shown to behave identically upon ion exchange chromatography to the AFP in a mixture of sera from boar and newborn pigs. The assay described provides a basis for determining correlations between AFP levels in fetal-maternal compartments and fetal survival and maturity in swine.
doi:10.1095/biolreprod20.4.947 pmid:88234 fatcat:ydeh5wzjcnf5leehw7geooieqa