Previous investigations (Winzler, 1944; Winzler et al., 1944; Spiegelman, 1947) have skown that sodium azide can dissociate anaerobic glycolysis from a wide variety of biosyntheses without impairing the rate of fermentation. In addition, azide was found (Spiegelman et al., 1948) to prevent net phosphate esterification during fermentation, to decrease the sensitivity of the fermentation to monoiodoacetic acid and other poisons of triose phosphate dehydrogenase, and to increase the sensitivity to

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... fluoride which poisons enolase. These effects became apparent at the same concentrations of azide that could stop biosynthesis. The results were made explicable by the supposition (Spiegelman et al., 1948) that azide can, by replacement, destroy the energy rich acyl phosphate bonds generated at the level of triose phosphate dehydrogenase activity.