Oocyte Selection for in vitro embryo Production in Bovine Species: Non-Invasive Approaches for the New Challenges of Oocyte Competence [post]

Luis Aguila, Favian Treulen, Jacinthe Therrien, Ricardo Felmer, Martha Valdivia, Smith Lawrence C
2020 unpublished
The efficiency of producing embryos using in vitro technologies in livestock species rarely exceeds the 30 to 40% threshold, indicating that the proportion of oocytes that fail to develop after in vitro fertilization and culture is considerably large. Considering that the intrinsic quality of the oocyte is one of the main factors affecting blastocyst yield, the precise identification of non-invasive cellular or molecular markers that predict oocyte competence is of major interest to research
more » ... practical applications. The aim of this review was to explore the current literature on different non-invasive markers associated with oocyte quality in the bovine model. Apart from some controversial findings, the presence of cycle-related structures in ovaries, a follicle size between 6 and 10 mm, large number of surrounding cumulus cells, slightly expanded investment without dark areas, large oocyte diameter (>120 microns), dark cytoplasm, and the presence of a round and smooth first polar body have been associated to better competence. In addition, the combination of oocyte and zygote selection by BCB test and spindle imaging have the potential to further optimize the identification of oocytes with better developmental competence for in vitro-derived technologies in livestock species.
doi:10.20944/preprints202010.0453.v2 fatcat:6wfloslmwne7blt3dbxqkvocju