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A global proteomics strategy was initiated to decipher molecular mechanisms associated with the blood-brain barrier (BBB) phenotype of the brain capillary endothelial cells. The different methods implemented were shown complementarily. The main results obtained using an in vitro BBB model allowed highlighting the role of several protein actors of cytoskeleton remodelling, the involvement of the asymmetric dimethylarginine pathway in regulating endothelial function and the role of tissuedoi:10.4236/jbm.2014.21003 fatcat:gmxh2l6kbnbjdda4vmflyd3idm