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Evaluation of methods for whole genome sequencing of Enterococcus faecium in a diagnostic laboratory
[article]
2021
medRxiv
pre-print
Enterococcus faecium is an important nosocomial pathogen associated with hospital transmission and outbreaks. Based on growing evidence that bacterial whole genome sequencing enhances hospital outbreak investigation of other bacterial species, our aim was to develop and evaluate methods for low volume clinical sequencing of E. faecium. Using a test panel of 22 E. faecium isolates associated previously with hospital transmission, we developed laboratory protocols for DNA extraction and library
doi:10.1101/2021.11.09.21265457
fatcat:hxxhj7illveshpevcy2if2cecq