Objectives: Mistletoes have benefits for the treatment of human and animal health ailments. This study investigated the physicochemical and phytochemical constituents of Englerina woodfordioides, Phragmanthera regularis, Tapinanthus globiferus, and Viscum tuberculatum from central Ethiopia. Results: The four plant species collected from 11 host trees grown in six distinct locations revealed the presence of alkaloids, phenols, saponins, cardiac glycosides, steroids, terpenoids, tannins,

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... and coumarins. In contrast, no flavonoids, anthraquinones and phlobatannins were detected. The total ash value of E. woodfordiodes and P. regularis ranges from 6.04 to 13.23% and 5.62 to 15.22%, respectively. Comparable total ash content was found in T. globiferus and V. tuberculatum. However, no significant difference was obtained in the mean percent moisture content of the study species.