Structural and Functional Analysis of Multi-Interface Domains

Liang Zhao, Steven C. H. Hoi, Limsoon Wong, Tobias Hamp, Jinyan Li, Jens Kleinjung
2012 PLoS ONE  
A multi-interface domain is a domain that can shape multiple and distinctive binding sites to contact with many other domains, forming a hub in domain-domain interaction networks. The functions played by the multiple interfaces are usually different, but there is no strict bijection between the functions and interfaces as some subsets of the interfaces play the same function. This work applies graph theory and algorithms to discover fingerprints for the multiple interfaces of a domain and to
more » ... ablish associations between the interfaces and functions, based on a huge set of multi-interface proteins from PDB. We found that about 40% of proteins have the multi-interface property, however the involved multi-interface domains account for only a tiny fraction (1.8%) of the total number of domains. The interfaces of these domains are distinguishable in terms of their fingerprints, indicating the functional specificity of the multiple interfaces in a domain. Furthermore, we observed that both cooperative and distinctive structural patterns, which will be useful for protein engineering, exist in the multiple interfaces of a domain.
doi:10.1371/journal.pone.0050821 pmid:23272073 pmcid:PMC3522720 fatcat:egooznsze5b7hcmytzxyq423y4