Escherichia coli Mutant with Elevated Nicotinamide Adenine Dinucleotide Phosphate (Reduced) Oxidase Activity

Laurence M. Corwin, G. Richard Fanning
1969 Journal of Bacteriology  
have been described (2). NADPH and reduced nicotinamide adenine dinucleotide (NADH) oxidases were determined by following the decrease in absorbancy at 340 nm. The assays were made in 0.05 M tris(hydroxymethyl)aminomethane (Tris) buffer (pH 7.5) or in 0.05 M cacodylate buffer (pH 6.0). Pyridine nucleotide transhydrogenase was assayed by following the reduction of acetylpyridinenicotinamide adenine dinucleotide (NAD) by the method of Stein et al. (9) . All kinetic experiments were conducted in a
more » ... were conducted in a Gilford 2000 recording spectrophotometer equipped with an automatic cuvette positioner and auxilliary offset control. Oxygen uptake was measured in a Warburg respirometer at 37 C, with substrates at 1362 on May 9, 2020 by guest
doi:10.1128/jb.97.3.1362-1373.1969 fatcat:tbgrza2uvbhjfdiarywkrdjowa