Imaging of cardiovascular structures using near-infrared femtosecond multiphoton laser scanning microscopy

Katja Schenke-Layland, Iris Riemann, Ulrich A. Stock, Karsten König
2005 Journal of Biomedical Optics  
Multiphoton imaging represents a novel and very promising medical diagnostic technology for the high-resolution analysis of living biological tissues. We performed multiphoton imaging to analyzed structural features of extracellular matrix (ECM) components, e.g., collagen and elastin, of vital pulmonary and aortic heart valves. Highresolution autofluorescence images of collagenous and elastic fibers were demonstrated using multifluorophore, multiphoton excitation at two different wavelengths
more » ... rent wavelengths and optical sectioning, without the requirement of embedding, fixation, or staining. Collagenous structures were selectively imaged by detection of second harmonic generation (SHG). Additionally, routine histology and electron microscopy were integrated to verify the observed results. In comparison with pulmonary tissues, aortic heart valve specimens show very similar matrix formations. The quality of the resulting three-dimensional (3-D) images enabled the differentiation between collagenous and elastic fibers. These experimental results indicate that multiphoton imaging with near-infrared (NIR) femtosecond laser pulses may prove to be a useful tool for the nondestructive monitoring and characterization of cardiovascular structures. © 2005 Society of Photo-Optical Instrumentation Engineers.
doi:10.1117/1.1896966 pmid:15910091 fatcat:eushu5x2xngvvid7xlfp5cqcg4