The present study deals with the isolation of E. coli causing Urinary Tract Infection among pregnant women, its antibiotic resistance pattern and to find the association between the risk factors contributing towards the occurrence of infections. A total of 300 urine samples were collected from pregnant ladies visiting for their antenatal checkup in different hospitals of Lahore. Details of patients regarding age, socioeconomic status, personal hygiene, previous history of Urinary Tract

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... , trimester of pregnancy, habit of retaining urine, history of diabetes, history of catheterization, history of symptoms of Urinary Tract Infection and frequency of sexual activity were noted. Bacteria were isolated and biochemically identified. Antibiotic sensitivity pattern of E. coli was tested by using Kirby Bauer disc diffusion method. Out of 300 urine samples, 69 showed positive growths for Escherichia coli and it was the most common pathogen. The prevalence of Urinary Tract Infection was 23%. E. coli showed 73.91%