Isolation and Characteristics of Microorganisms Producing Extracellular Enzymes from Jeju Traditional Fermented Soybean Paste (Doenjang)
제주전통된장으로부터 세포외효소 분비능이 우수한 미생물의 분리 및 특성

You-Sung Oh, Ji-Eun Park, Hyun-Jeong Oh, Jung-Hyon Kim, Myung-Cheol Oh, Chang-Kyung Oh, Young-Ju Oh, Sang-Bin Lim
2010 Journal of the Korean Society of Food Science and Nutrition  
Bacteria strains with high activities of extracellular enzymes (protease, fibrinolytic enzyme, amylase, cellulase, and lipase) were isolated from Jeju traditional fermented soybean paste (Doenjang), and characterized by 16S rRNA gene sequence analysis and physiological properties. Protease activities were higher in JR14, JR19, JR25, JR32, JR38, JR47, and JR64 than Bacillus subtilis KCCM 12027 (standard strain). Amylase activities were shown in JR6, JR25, JR38, JR56 and JR81, while not in
more » ... 27. Cellulase activities were higher in JR6, JR14, JR48, and JR65 than those of other isolated strains and KCCM 12027 whereas lipase activities were the higher in JR-14 and JR-48. Thrombolytic activity in JR19 with high hemolysis activity were 192% compared with that of plasmin as a positive control. Zymogram analysis indicated that the thrombolytic active strains had 4~5 bands in the molecular weight range of 25~75 kDa. Gene sequence analysis of rRNA revealed that the isolated stains had 99% homology with Bacillus species, and the thrombolytic active stain JR19 was B. stratosphericus 41KF2a T .
doi:10.3746/jkfn.2010.39.1.047 fatcat:wmvo3b3zl5ezrjx6bza2mjx4te