Effect Inoculant Mixed Culture On Theobromine Cocoa Pod Silage (Page 226-229)

Muhammad Askari Zakariah, M Zakariah
2017 Zenodo  
The objective of this study was to identify the effect of L. plantarum and S. cerevisiae inoculated into cocoa pod silage on theobromine concentration. This experiment consists of four treatments: 1) 1 kg freshly harvested cocoa pods without inoculant as control (K); 2) inoculated with L. plantarum (KLp); 3) inoculated with S. cerevisiae (KSc); and 4) inoculated with L. plantarum and S. cerevisiae mixture (KLp+Sc), and adding cassava meal as additive. Each treatment was replicated 3 times, and
more » ... hen was fermented for 21 days. Variables observed were theobromine concentration. Collected data of theobromine concentration was analyzed by one-way analysis of variance and followed by Duncan's new multiple range test (DMRT), if there were any significant difference. Results showed that treatment effect was significant (P<0.05). Content of theobromine of cocoa pod freshly was 161.28 ppm. Cocoa pod silage inoculated with L. plantarum and S. cerevisiae mixed culture had lower (P<0.05) theobromine concentration than cocoa pod silage with no inoculant (3.88 ppm vs. 7.80 ppm, respectively). Based on this result we can conclude that inoculants L. plantarum and S. cerevisiae can reduce theobromine concentration on cocoa pod silage and that is safety when used to feed cattle.
doi:10.5281/zenodo.1434030 fatcat:fp5ohjyhgvf7rpfnubs7wlapie