Amino Acid Sequences of Ferredoxins from Atropa belladonna and Hyoscyamus niger: Their Similarities to Those in Other Tropane-Alkaloid-Containing Plants

Yoshiki Mino, Mayumi Yukita, Nobuhiro Hiratsuka, Hiroyuki Wariishi
2005 Biological and Pharmaceutical Bulletin  
The complete amino acid sequences of [2Fe-2S] ferredoxin from Atropa belladonna and Hyoscyamus niger have been determined by automated Edman degradation of the entire S-carboxymethylcysteinyl proteins and of the peptides obtained by enzymatic digestion. These two ferredoxins exhibited 1-8 differences in their amino acid sequences compared to those of other tropane-alkaloid-containing plants (Scopolia japonica, Datura stramonium, D. metel, and D. arborea), and only 1 or 4 differences compared to
more » ... S. japonica and D. arborea. In contrast, 9-23 differences were observed among the other solanaceous ferredoxins. This suggests that tropane-alkaloidcontaining plants are closely related taxonomically.
doi:10.1248/bpb.28.1535 pmid:16079510 fatcat:37hxxx77h5elvdtvsixhhai25i