Pylephlebitis Due to Parvimonas micra

Takashi Shinha, Virginia Caine
2016 Infectious Diseases in Clinical Practice  
Parvimonas micra is Gram-positive, strict anaerobic, nonmotile, and non-spore forming coccus. It is a member of oral flora and is related to oral infectious diseases as well as systemic diseases. P. micra KCOM 1037 (= ChDC B276) was isolated from human postoperative maxillary cyst lesion. Here, we present the complete genome sequence of P. micra KCOM 1037. Parvimonas micra (formerly Peptostreptococcus micros) is a Gram-positive, strict anaerobic, non-motile, and non-spore forming coccus
more » ... ming coccus (Murdoch and Shah, 1999; Tindall and Euzéby, 2006) . It is a member of oral flora and is related to oral infectious diseases (Haffajee and Socransky, 1994; de Sousa et al., 2003) as well as systemic diseases (Murdoch et al., 1988; Civen et al., 1995; Bartz et al., 2005; Endo et al., 2015; Gomez et al., 2015) . Parvimonas micra KCOM 1037 (= ChDC B276) was isolated from human postoperative maxillary cyst lesion. In this report, we presented the complete genome sequence of P. micra KCOM 1037. The P. micra KCOM 1037 was grown in a tryptic soy broth (TSB, Difco Laboratories) medium supplemented with 0.5% yeast extract, 0.05% cysteine HCl-H2O, 0.5 mg/ml of hemin, 2 µg/ml of vitamin K1, and 5% sheep blood in an anaerobic chamber (Model Bactron I) maintained using a gas mixture of 10% H2, 5% CO2, and 85% N2 (Park and Kook, 2013). The bacterial genomic DNA was prepared as previously described (Cho et al., 2015). Genomic DNA of P. micra KCOM 1037 was sequenced using PacBio RSII SMRT sequencing platform using a 20 kb SMRTbell template library and Illumina HiSeq platform with 100 × 2 bp reads using 350 bp insert size library by Macrogen Inc. Approximately 777.3 Mb (457.7 ×) with 153,365 filtered subreads (mean subreads length: 5,068 bp) were generated and assembled into a single contig by HGAP (version: 3.0, default setting) in PacBio's SMRT portal (http://www.pacb.com/products-and-services/analytical-soft ware/smrt-analysis). The initial assembly was polished by Pilon (version: 1.21) with 1,561.6 Mb paired-end reads (939.7
doi:10.1097/ipc.0000000000000286 fatcat:ksyf636gyvgrhbkkcw77zxdi6q