ISOLATIO OF BIOSURFACTAT PRODUCIG BACTERIA FROM EVIROMETAL SAMPLES

R Jaysree, Subham Basu, Priyanka Singh, Twinkle Ghosal, Pragya Patra, Yekala Keerthi, Rajendran, N Rajendran
unpublished
Biosurfactants are amphiphilic compounds produced extracellularly by microorganisms on cell surfaces, or excreted extracellularly. They contain hydrophilic and hydrophobic moieties that reduce surface and interfacial tension between molecules at the surface and interface respectively. The present study was focused on isolation of biosurfactant producing bacteria from environmental samples (contaminated soil and uncontaminated water) and assessing the potential of these isolates by various
more » ... es by various standard method. Oil displacement and emulsification index methods were used to screen the capability of isolates for producing biosurfactant. Studies were also carried out using diesel and engine oil as hydrocarbon source. The identified bacteria Bacillus subtilis and Bacillus cereus which were isolated from water and soil respectively were found to possess biosurfactant producing capabilities. The biosurfactants produced by B. subtilis had an emulsification capacity (E 24) of 20% and 15%, and that by B. cereus was 30% and 20% for diesel and engine oil respectively. Results of the present study suggested that these isolates have potential to use in oil degradation studies.
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