The identification of insect species is an important key in forensic entomology. Molecular examination of the nucleotide sequences of cytochrome c oxidase subunit II (COII) gene has been widely performed before the morphology identification. We analyzed the partial conserved COII nucleotide sequence of the forensic related flies. After the DNA extraction from collected larvae, thus PCR amplification of conserved sequencing of the partial COII sequence was implemented. Obtained sequences were

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... lyzed for a phylogenetic tree and a distance matrix. Our data showed a very low intraspecific sequence distance among Calliphoridae and Sarcophagidae to the flies' species of Chrysomyia megacephala. Further research should attempt to determine the larval growth of newly discovered flies, to apply the time of death in forensic crime setting.