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Automated Microdensitometry And Protein Assays As Measures For Platelet Adhesion And Aggregation On Collagen Coated Slides Under Controlled Flow Conditions
1979
VIIth International Congress on Thrombosis and Haemostasis
unpublished
Microscope slides were homogeneously coated over a length of 2 cm with a mixture of soluble and fibrillar collagen and exposed at 37°C and under laminar flow to citrated whole rabbit blood at a flow-rate of 100 ml/min. Surface coverage with platelets (adhesion) and platelet accumulations higher than about 5 μm in height (aggregation) were determined by automated microdensitometry of fuchsine stained 'en face' preparations. The platelet mass per unit surface was measured with a modified Lowry
doi:10.1055/s-0038-1665758
fatcat:xu53bymf45ay5pxtlfsqjl5kbm