Key words: antimicrobial activity, Boswellia ovalifoliolata Plants produce a wide variety of phyto chemical constituents, which are secondary metabolites and are used either directly or indirectly in pharmaceutical industry. Medicinal plants play an important role in health care. There are number of plants used for treating diseases which are not comprehensively documented due to a lack of communication and low frequency of their use. Such a group of medicinal plants are some endemic plants of

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... irumala-Tirupati. These plants have medicinal properties and are used in herbal therapy to cure a wide range of health problems. There is a need to bridge the gap for data access on these plants to the scientific community. Very small amount of work has been down on these plants and a lot is to be done. We select endemic medicinal plants of Tirupati flora boswellia ovalifoliolata, member of a burseraceae family and grow in subtropical temperature. This study was intended to evaluate the anti-bacterial activity ethonol extracts of boswellia ovalifoliolata stem bark in gram positive and gram negative bacteria. ethnol extracts exhibited significant antibacterial activity, which supports the traditional medicinal utilization of the plant. This study established anti-bacterial activity of Stem bark of Boswellia ovalifoliolata. INTRODUCTION: