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<a target="_blank" rel="noopener" href="https://fatcat.wiki/container/s2lvgbtkfnfaha7wzlrwo7epnq" style="color: black;">Surgical neurology international</a>
Gene sequencing has played an integral role in the advancement and understanding of disease pathology and treatment. Although historically expensive and time consuming, new sequencing technologies improve our capability to obtain the genetic information in an accurate and timely manner. Within neurosurgery, gene sequencing is routinely used in the diagnosis and treatment of neurosurgical diseases, primarily for brain tumors. This paper reviews nanopore sequencing, an innovation utilized by<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.4103/sni.sni_55_18">doi:10.4103/sni.sni_55_18</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/30159201">pmid:30159201</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC6094492/">pmcid:PMC6094492</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/54goaewnivgvzitr3fl26c5yvq">fatcat:54goaewnivgvzitr3fl26c5yvq</a> </span>
more »... N and outlines its potential use for neurosurgery. Methods: A literature search was conducted for publications containing the keywords of Oxford MinION, nanopore sequencing, brain tumor, glioma, whole genome sequencing (WGS), epigenomics, molecular neuropathology, and next-generation sequencing (NGS). In total, 64 articles were selected and used for this review. Results: The Oxford MinION nanopore sequencing technology has had successful applications within clinical microbiology, human genome sequencing, and cancer genotyping across multiple specialties. Technical details, methodology, and current use of MinION sequencing are discussed through the prism of potential applications to solve neurosurgery-related scientific and diagnostic questions. The MinION device has proven to provide rapid and accurate reads with longer read lengths when compared with NGS. For applications within neurosurgery, the MinION device is capable of providing critical diagnostic information for central nervous system (CNS) tumors within a single day. Conclusions: MinION provides rapid and accurate gene sequencing with better affordability and convenience compared with current NGS methods. Widespread success of the MinION nanopore sequencing technology in providing accurate, How to cite this article: Patel A, Belykh E, Miller EJ, George LL, Martirosyan NL, Byvaltsev VA, et al. MinION rapid sequencing: Review of potential applications in neurosurgery. Surg Neurol Int 2018;9:157. http://surgicalneurologyint.com/MinION-rapid-sequencing:-Review-of-potential-applications-in-neurosurgery/ This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. For reprints contact: firstname.lastname@example.org Access this article online Website: www.surgicalneurologyint.com
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