Identification of a New Pheromone-Binding Protein in the Antennae of a Geometrid Species and Preparation of Its Antibody to Analyze the Antennal Proteins of Moths Secreting Type II Sex Pheromone Components

Hayaki WATANABE, Hiroko TABUNOKI, Nami MIURA, Aya MATSUI, Ryoichi SATO, Tetsu ANDO
2009 Bioscience, biotechnology and biochemistry  
The full-length cDNA sequence of a new pheromonebinding protein (AscrPBP2) was determined from a geometrid moth, Ascotis selenaria cretacea, which secreted a Type II sex pheromone, and an antiserum against its recombinant protein overexpressed in Escherichia coli was prepared. In addition to this antiserum against AscrPBP2, antibodies against AscrPBP1 and general odorant-binding proteins of Bombyx mori were used in Western blotting experiments to analyze the proteins in the antennae of several
more » ... epidopteran species secreting Type II sex pheromone components.
doi:10.1271/bbb.90029 pmid:19502757 fatcat:jcqf7unx6zevxfks7555p7joqe