Comparative Genomic Hybridization Analysis of Nonfunctioning Pituitary Tumors

M. Daniely
1998 Journal of Clinical Endocrinology and Metabolism  
Clinically nonfunctioning pituitary adenomas constitute about one third of pituitary neoplasms and are considered monoclonal tumors. The molecular mechanisms of tumorigenesis in these neoplasms are poorly understood, as evidenced by the paucity of reported somatic genetic alterations. Furthermore, the somatic mutations detected to date were primarily ascribed to candidate genes or chromosomal regions: gsp, ras, p53 mutations, and allelic losses of 11q and 13q. To gain insight into which
more » ... mal regions bear genes involved in nonfunctioning pituitary tumorigenesis, we examined 23 such tumors by comparative genomic hybridization. Four tumors showed no genetic abnormality, and the rest (17 of 23, 74%) exhibited at least one All tumors were examined by a neuropathologist using separate formalin-fixed, paraffin-embedded, hematoxylin-eosin-stained sections. All immuncytochemical studies were performed using a standard peroxidase-antiperoxidase method (18) with rabbit antibodies against PRL,
doi:10.1210/jc.83.5.1801 pmid:9589696 fatcat:ssaaflk3ybdajp3457nllrqnm4