AAV-PHP.S-mediated delivery of reporters to cranial ganglion sensory neurons [article]

Andoni I Asencor, Gennady Dvoryanchikov, Pantelis Tsoulfas, Nirupa Chaudhari
2021 bioRxiv   pre-print
Because of their ease of use and low risk containment, Adeno-Associated Virus vectors are indispensable tools for much of neuroscience. Yet AAVs have been used relatively little to study the identities and connectivity of peripheral sensory neurons because methods to selectively target particular receptive fields or neuron types have been limited. The introduction of the AAVPHP.S capsid with selective tropism for peripheral neurons (Chan et al., 2017) offered a solution, which we further
more » ... te here. We demonstrate using AAV-PHP.S with GFP or mScarlet reporters, that all cranial sensory ganglia, i.e. for cranial nerves V, VII, IX and X, are targeted. Pseudounipolar neurons of both somatic and visceral origin, but not satellite glia, are efficiently transduced rapidly and express the gene of interest within 1 week of injection. Fluorescent reporter proteins are transported into the central and peripheral axons of these sensory neurons, permitting visualization of terminals at high resolution, and/or in intact, cleared brain using light sheet microscopy. By combining a Cre-dependent reporter with the AAVPHP.S capsid, we confirmed expression in a cell-type dependent manner for both anatomical and targeted functional analyses. The AAV-PHP.S capsid will be a powerful tool for mapping the receptive fields and circuits of molecular subtypes of many somatosensory, gustatory and visceral sensory neurons.
doi:10.1101/2021.09.14.460327 fatcat:us73czl3r5hrlc7tfjaoyk3lci