A multispectral microscope for in vivo oximetry of rat dorsal spinal cord vasculature

Marieke A van der Putten, Lewis E MacKenzie, Andrew L Davies, Javier Fernandez-Ramos, Roshni A Desai, Kenneth J Smith, Andrew R Harvey
2017 Physiological Measurement  
2017) 'A multispectral microscope for in vivo oximetry of rat dorsal spinal cord vasculature.', Physiological measurement., 38 (2). p. 205. The full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-prot purposes provided that: • a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed
more » ... in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders. Please consult the full DRO policy for further details. Abstract 13 Quantification of blood oxygen saturation (SO2) in vivo is essential for understanding the 14 pathogenesis of diseases in which hypoxia is thought to play a role, including inflammatory 15 disorders such as multiple sclerosis (MS) and rheumatoid arthritis (RA). We describe a low-cost 16 multispectral microscope and oximetry technique for calibration-free absolute oximetry of 17 surgically exposed blood vessels in vivo. We imaged the vasculature of the dorsal spinal cord in 18 healthy rats, and varied inspired oxygen (FiO2) in order to evaluate the sensitivity of the imaging 19 system to changes in SO2. The venous SO2 was calculated as 67.8 ± 10.4% (average ± standard 20 deviation), increasing to 83.1 ± 11.6% under hyperoxic conditions (100% FiO2) and returning
doi:10.1088/1361-6579/aa5527 pmid:28001129 fatcat:rz7aay23y5fzzdqo6xqxjmtreu