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Ojectives: To explore the effect of STAT3 pathway on the autophagy of human glomerular mesangial cells (HGMC) and expression of extracellular matrix Collagen IV cultured in high glucose. Methods: HGMC were cultured in normal (5 mmol/L) or high glucose (30 mmol/l) for 12e72 hours, and then S3I-201 (an inhibitor of STAT3 activity) or STAT3-siRNA transfection were added into HGMC. STAT3, p-STAT3 and autophagy marker proteins LC3 and p62 protein expression was evaluated by western blot,doi:10.1016/j.hkjn.2015.08.051 fatcat:fk4p3yqjabcdhf45fp32ucpsai