Altered MicroRNA Expression in Cervical Carcinomas

J.-W. Lee, C. H. Choi, J.-J. Choi, Y.-A. Park, S.-J. Kim, S. Y. Hwang, W. Y. Kim, T.-J. Kim, J.-H. Lee, B.-G. Kim, D.-S. Bae
2008 Clinical Cancer Research  
Purpose: MicroRNAs (miRNA) are small noncoding RNAs that are 18 to 25 nucleotides in length; they regulate the stability or translational efficiency of target mRNAs. Emerging evidence suggests that miRNAs might be involved in the pathogenesis of a variety of human cancers. Experimental Design: In this study, we profiled miRNA expression in 10 early stage invasive squamous cell carcinomas (ISCC) and 10 normal cervical squamous epithelial specimens using TaqMan real-time quantitative PCR array
more » ... tative PCR array methods. In order to evaluate the role of miR-199a, one of the most significantly overexpressed in ISCCs, we transfected cervical cancer cells (SiHa and ME-180) with anti^miR-199a oligonucleotides and assessed the cell viability. Results: We found 70 genes (68 up-regulated, 2 down-regulated) with significantly different Fig. 2 . Real-time quantitative PCR analysis of the mRNA level of DNM2 intron 16, which is the host gene of miR-199a ; the mRNA level was significantly higher in ISCCs (P < 0.0001).
doi:10.1158/1078-0432.ccr-07-1231 pmid:18451214 fatcat:b2bmy3rfyfbfhhgrjs32cy5ova