DEVELOPMENT AND VALIDATION OF REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR ESTIMATION OF NICOTINE IN NICOTINE GUM TABLET

Patel Jignesh, Patel Dipti
2014 unpublished
This research manuscript describes simple, sensitive, accurate, precise and repeatable reverse phase high performance liquid chromatography method for the determination of nicotine in gum tablet. The sample was analyzed by reverse phase C 18 column (Phenomenex C 18 , 250 mm × 4.6 mm, 5µ) as stationary phase; acetonitrile : phosphate buffer (PH-3.5) (70 : 30 , v/v) as a mobile phase at a flow rate of 1.0 ml/min. Quantification was achieved with Photo Diode Array detector at 260 nm. The retention
more » ... 0 nm. The retention time for nicotine was found to be 3.5 min. The linearity was obtained in the concentration range of 0.2-1.2 µg/ml for nicotine. The method was successfully applied to gum tablet dosage form because no chromatographic interferences from formulation excipients were found. The method retained its accuracy and precision when the standard addition technique was applied.
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