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Gene Transfer in Marine Water Column and Sediment Microcosms by Natural Plasmid Transformation
1991
Applied and Environmental Microbiology
We investigated the possibility for natural transformation in the marine environment by using broad-hostrange plasmid multimers and a high-frequency-of-transformation (HFT) Vibrio strain as the recipient. Water and sediment samples were taken from Tampa Bay, the eastern Gulf of Mexico, the Florida Shelf near Miami, and the Bahamas Bank. In water column microcosms, transformation frequencies ranged from 1.7 x 10-6 to 2.7 x 10-10 transformants per recipient, with highest frequencies occurring
doi:10.1128/aem.57.5.1509-1515.1991
fatcat:dhsselqeh5g3rgvzfviqokqfzy