Prevalence of human herpesvirus 6A and 6B during pregnancy

Beáta Mészáros, Tamás Sápy, Lajos Gergely, Eszter Csoma
2014 Acta Microbiologica et Immunologica Hungarica  
The aim of the present study was to assess the frequency of human herpesvirus 6A (HHV-6A) and human herpesvirus 6B (HHV-6B) infection during pregnancy. 100-100 blood samples were collected from pregnant and non pregnant women, then nucleic acid was isolated from both plasma and leukocytes fraction. Nested and real-time PCR were used to detect and differentiate HHV-6A and HHV-6B DNA and to determine viral loads. Reverse transcription PCR (RT-PCR) for HHV-6 U79/80 mRNA was performed in order to
more » ... ormed in order to reveal active HHV-6 replication. HHV-6A and HHV-6B active infection was not detected in blood samples neither from pregnant nor from non pregnant women. Frequency of HHV-6B and HHV-6A latency did not show difference between the studied groups (15 % vs. 16%). HHV-6B latency was dominant in both studied groups (14/15 and 15/16). Beside these results, in leukocyte samples of one pregnant and three non pregnant women high HHV-6A viral loads (1.28 X 10 5 -5.07 X 10 5 GEq / 1.5 X 10 6 leukocytes) were detected, and viral DNA was also found in plasma samples. Although RT-PCR did not confirm virus replication, but chromosomal integration was also not proved unequivocally, the number of 0.08-0.33 HHV-6 copy/ 1 leukocyte refers more to postnatal infection.
doi:10.1556/amicr.61.2014.3.5 pmid:25102917 fatcat:iz6byik5azdbjnado52mh5gaa4