Full Text] [Abstract] , November 1, 2000; 68 (5): 587-592. J. Leukoc. Biol. S. Böckmann and I. Paegelow Kinins and kinin receptors: importance for the activation of leukocytes [PDF] [Full Text] [Abstract] , November 1, 2002; 283 (5): F934-F943. Am J Physiol Renal Physiol and J. M. Saavedra Estrogen upregulates renal angiotensin II AT2 receptors [PDF] [Full Text] [Abstract] , December 1, 2002; 62 (6): 1344-1355. Mol. Pharmacol. Silencer Element Transcriptional Regulation of the Rat Bradykinin B2

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... Receptor Gene: Identification of a [PDF] [Full Text] [Abstract] , February 1, 2006; 290 (2): F456-F464. Am J Physiol Renal Physiol R. Zamorano, S. Suchindran and J. V. Gainer expression 3'-Untranslated region of the type 2 bradykinin receptor is a potent regulator of gene on the following topics: respective cells and vasculature, as well as to the control of body fluid volume and composition. It is published 12 times a year publishes original manuscripts on a broad range of subjects relating to the kidney, urinary tract, and their AJP -Renal Physiology kinin receptor upregulation by cAMP is associated with BK-induced PGE 2 production in rat mesangial cells. Am. J. Physiol. 274 (Renal Physiol. 43): F532-F540, 1998.-In the rat mesangial cell (MC), activation of the bradykinin B 2 receptor (B 2 R) by bradykinin (BK) is associated with both phospholipase C (PLC) and A 2 (PLA 2 ) activities and with inhibition of adenosine 3Ј,5Ј-cyclic monophosphate (cAMP) formation leading to cell contraction. Because cAMP plays an important role in the regulation of gene expression in general, we investigated the effect of increasing the intracellular cAMP concentration ([cAMP] i ) in mesangial cells on the B 2 mRNA expression, on the density of B 2 receptor binding sites, on the BK-induced increase in both the free cytosolic Ca 2ϩ concentration ([Ca 2ϩ ] i ), and in the prostaglandin E 2 (PGE 2 ) production. Forskolin, PGE 2 , and cAMP analog, 8-bromoadenosine 3Ј,5Ј-cyclic monophosphate (8-BrcAMP), were used to increase [cAMP] i . Twenty-four-hour treatment with forskolin, PGE 2 , and 8-BrcAMP resulted in significant increases in B 2 receptor binding sites, which were inhibited by cycloheximide. The maximum B 2 receptor mRNA expression (160% above control) was observed in cells treated during 24 h with forskolin and was prevented by actinomycin D. In contrast, the D-myo-inositol 1,4,5-trisphosphate (IP 3 ) formation and the BK-induced increase in [Ca 2ϩ ] i , reflecting activation of PLC, were not affected by increased levels of [cAMP] i . However, the BK-induced PGE 2 release, reflecting PLA 2 activity, was significantly enhanced. These data bring new information regarding the dual signaling pathways of B 2 receptors that can be differentially regulated by cAMP. We thank Dr. P. Winterton for revising the English version. This work was partly funded by grant from région Midi-Pyrenées (RECH/9407562). M. E. Marin Castañ o is supported by a grant from the French Nephrology Society. J. P. Schanstra is recipient of a postdoctoral fellowship position