Use of a Mycoplasma suis-PCR protocol for screening a population of captive peccaries (Tayassu tajacu and Tayassu pecari)

Rafael Felipe da Costa Vieira, Marcelo Beltrão Molento, Ana Marcia Sá Guimarães, Andrea Pires dos Santos, Marcelo Bonat, Manoel Lucas Javorouski, Luciene Popp, Leonilda Correia dos Santos, Wanderlei Moraes, Zalmir Silvino Cubas, Thállitha Samih Wischral Jayme Vieira, Odilon Vidotto (+3 others)
2011 Revista Brasileira de Parasitologia Veterinária  
Mycoplasma suis is a hemotropic bacteria of red blood cells and the causative agent of swine eperythrozoonosis. Diagnosis of infection may be reached by direct examination of blood smears; however, the use of polymerase chain reaction (PCR) of the 16S RNA gene of M. suis improves the sensitivity and specificity of detection. The aim of this study was to screen peccaries (Tayassu tajacu and T. pecari) for M. suis infection using a specific conventional PCR. A total of 28 blood samples from
more » ... e collared and white-lipped peccaries were collected, DNA extracted and a specific M. suis PCR assay performed. All samples were negatives by both blood smear examination and PCR testing. To verify the presence of amplifiable DNA, PCR for beta-actin gene was performed in all samples. This study was part of an active surveillance program, which is crucial for monitoring animal health status, particularly in wildlife species.
doi:10.1590/s1984-29612011000100016 pmid:21439238 fatcat:zrbwyaan5rb7fmmf23aaunuebq