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Methods and Applications of Campenot Trichamber Neuronal Cultures for the Study of Neuroinvasive Viruses
The development of compartmentalized neuron culture systems has been invaluable in the study of neuroinvasive viruses, including the alpha herpesviruses Herpes Simplex Virus 1 (HSV-1) and Pseudorabies Virus (PRV). This chapter provides updated protocols for assembling and culturing rodent embryonic superior cervical ganglion (SCG) and dorsal root ganglion (DRG) neurons in Campenot trichamber cultures. In addition, we provide several illustrative examples of the types of experiments that aredoi:10.20944/preprints202105.0098.v1 fatcat:hil3takforae3ccx7ql6bqykwe