Axenic Culture of Myxamoebae of the Myxomycete Physarum polycephalum

E. M. Goodman
1972 Journal of Bacteriology  
Myxamoebae of the acellular slime mold Physarum polycephalum have been cultured axenically in a soluble medium. The growth medium contains bovine serum albumin, embryo extracts, liver infusion broth, peptone, and glucose. Cell densities ranging from 3 x 106 to 5 x 106 cells/ml have been obtained with this medium. To date, myxamoebae have been serially transferred more than 100 times without deleterious effect.
doi:10.1128/jb.111.1.242-247.1972 fatcat:6rbpgaszdjgi3g7pmlzd5vqida