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Patterns and kinetics of neurite extension from sympathetic neurons in culture are age dependent
Journal of Neuroscience
Long term (2-to 3-week) cultures of superior cervical ganglia (SCG) were established from rats and rat embryos ranging in age from 15 days of gestation (E15) to 279 days postnatal (P279). Cultures were grown on a collagen substratum and fed a serum-containing medium with added nerve growth factor. Radial outgrowth of neurites was measured as a function of time for up to 2 to 3 weeks. Computer-aided analysis generated estimates of onset time, initial rate, and subsequent changes in the rate ofdoi:10.1523/jneurosci.02-04-00503.1982 pmid:7069468 fatcat:cdrrs5lo7rbmvlqgkwje6xpc7a