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Changing Flux of Xylose Metabolites by Altering Expression of Xylose Reductase and Xylitol Dehydrogenase in Recombinant Saccharomyces cerevisiae
Biotechnology for Fuels and Chemicals
We changed the fluxes of xylose metabolites in recombinant Saccharomyces cerevisiae by manipulating expression of Pichia stipitis genes (XYL1 and XYL2) coding for xylose reductase (XR) and xylitol dehydroge nase (XDH), respectively. XYL1 copy number was kept constant by inte grating it into the chromosome. Copy numbers of XYL2 were varied either by integrating XYL2 into the chromosome or by transforming cells with XYL2 in a multicopy vector. Genes in all three constructs were under con trol ofdoi:10.1007/978-1-4612-0057-4_22 fatcat:qxejouojrzhcve4z7ur7fx33xm