Time-resolved imaging system for fluorescence-guided surgery with lifetime imaging capability

F. Powolny, K. Homicsko, R. Sinisi, Claudio E. Bruschini, E. Grigoriev, H. Homulle, John O. Prior, D. Hanahan, E. Dubikovskaya, E. Charbon, Jürgen Popp, Valery V. Tuchin (+3 others)
2014 Biophotonics: Photonic Solutions for Better Health Care IV  
We present a single-photon camera for fluorescence imaging, with a time resolution better than 100ps, capable of providing both intensity and lifetime images. the camera was fabricated in standard CMOS technology. With this FluoCam we show the possibility to study sub-nanosecond fluorescence mechanisms. The FluoCam was used to characterize a near-infrared probe, indocyanine green, conjugated with multimeric cyclic pentapeptide (cRGD). The fluorescent probe-conjugated was used to target and mark
more » ... to target and mark tumors with better specificity, in particular aiming at targeting the integrins α v β 3 and α v β 5 . As a first step towards clinical studies, preliminary results obtained in-vivo are presented. The first envisioned clinical application would be image-guided surgical oncology to help the surgeon to remove tumor tissue by a better discrimination from normal tissues and also to improve the detection of metastatic lymph nodes. A further application could be the in-vivo determination of the α v β 3 and α v β 5 targets to select patients for therapy with RGDchemotherapy conjugates. Downloaded From: http://proceedings.spiedigitallibrary.org/ on 12/01/2014 Terms of Use: http://spiedl.org/terms Proc. of SPIE Vol. 9129 912938-4 Downloaded From: http://proceedings.spiedigitallibrary.org/ on 12/01/2014 Terms of Use: http://spiedl.org/terms
doi:10.1117/12.2052411 fatcat:s4av7t5yzvhezddq2spxsiqtru