C 可 opreservation of jη 槻 畑 ・ grown Plant Me 臨 ems by Vit面 ca 甑on Toshikazu MATSUMOTO Complete vitrification can avoid the potentially damaging effectS of both extracellular and intracellular crystalliZation . This method is very usefu1 f()r long − term storage of gemlplasm due to minimum space and maintena 皿 ce requirement . Three cryogenic procedures ;vitrification , encapsulation − dehydratio 皿 and encapsUlation − vitrification , are suitable fbr cryopreservation of medsb 診ms or soma 廿c

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... os . h 曲 is paper, the ・ advantageous and disadvantageous between these procedures were evaluated and discussed . The vitrification method produces a high rate of shoot formation after cryopreservation , requires a minimaRime fbr dehydration , yet poses difficult when handling a large number of meristems during the treatment phase . The encapsulation − dehydration method is easy to handle and alleviates dehydration process, however , introduces a low survival rate . lt also produces a longer lag time for growth recovery and requires considerable time for dehydration . Contrarily , the encapsulation − vitrification method provides a high degree of survival , greatly shortens the dehydration time , and provides easy handling of even cells or small explants such as hairy rootS . 1