RAPID IN VITRO DIFFERENTIATION AND REGENERATION OF MOMORDICA DIOICA ROXB

Bimal Debnath, Sangram Sinha, Rabindra Sinha
2013 Online) An Online International Journal Available at   unpublished
High frequency in vitro regeneration and multiplication of shoots of female Momordica dioica Roxb. has been established from callus derived from nodal explants culture. Callus was established on Murashige and Skoog's (MS) agar gelled medium supplemented with 2mg/l 2, 4-D and 0.5mg/l BAP/ Coconut milk (15% v/v). Multiple shoot buds were developed from the callus when they were cultured on MS medium containing 4mg/l 6-Benzylaminopurine (BAP) and CM (15% v/v). Further shoot multiplication (86 ±
more » ... iplication (86 ± 3.44) was achieved by sub culturing of regenerated shoot clump on the same fresh medium. The shoots multiplication could be repeated for more than five cycles with normal morphogenesis without returning to the original explants source. For rooting, micro-propagated shoots were subsequently transferred to half strength MS medium containing 0.5mg/l Indole-3 butyric acid (IBA) with 100% success. Plants regenerated by organogenesis through callus are similar with the natural cytotypes. The present study highlights the rapid in vitro multiplication of M. dioica. Roxb.
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