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High frequency in vitro regeneration and multiplication of shoots of female Momordica dioica Roxb. has been established from callus derived from nodal explants culture. Callus was established on Murashige and Skoog's (MS) agar gelled medium supplemented with 2mg/l 2, 4-D and 0.5mg/l BAP/ Coconut milk (15% v/v). Multiple shoot buds were developed from the callus when they were cultured on MS medium containing 4mg/l 6-Benzylaminopurine (BAP) and CM (15% v/v). Further shoot multiplication (86 ±fatcat:zxt6gsiaufcwpm7p47jkdm443a