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Motivation: DNA methylation is an epigenetic mechanism of gene regulation. Bisulfite-conversion-based PCR methods, such as bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP), remain the most commonly used techniques for methylation mapping. Existing primer design programs developed for standard PCR cannot handle primer design for bisulfite-conversion-based PCRs due to changes in DNA sequence context caused by bisulfite treatment and many special constraints both on the primersdoi:10.1093/bioinformatics/18.11.1427 pmid:12424112 fatcat:oiw74tc3tfgrnk3o25gzi724uu