Chitinimonas prasina sp. nov., isolated from lake water

Y. Li, H. Zhu, Q. Lai, X. Lei, Z. Chen, H. Zhang, Y. Tian, W. Zheng, T. Zheng
2014 International Journal of Systematic and Evolutionary Microbiology  
A Gram-stain-negative, elongated rod-shaped, motile by gliding, green-pigmented, aerobic bacterial strain, designated LY03 T , was isolated from lake water in Xiamen, Fujian Province, China. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the isolate was a member of the genus Chitinimonas, which belongs to the family Burkholderiaceae. Strain LY03 T was most closely related to Chitinimonas taiwanensis LMG 22011 T (96.02 % 16S rRNA gene sequence similarity), followed by
more » ... nimonas koreensis KACC 11467 T (94.85 %), and the three strains formed a distinct lineage from other strains in the phylogenetic analyses. Optimum conditions for growth were 37 6C, pH 7-9 and without NaCl. The major fatty acids were summed feature 3 (C 16 : 1 v6c and/or C 16 : 1 v7c), C 16 : 0 and C 10 : 0 3-OH. The DNA G+C content of strain LY03 T was 63.6 mol% and the major respiratory quinone was ubiquinone-8 (Q-8). The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unknown polar lipids and unidentified phospholipids. Differential phenotypic properties and phylogenetic distinctiveness distinguished strain LY03 T from all other members of the genus Chitinimonas. On the basis of its morphology, physiology, fatty acid composition and 16S rRNA gene sequence data, strain LY03 T represents a novel species of the genus Chitinimonas, for which the name Chitinimonas prasina sp. nov. is proposed. The type strain is LY03 T (5MCCC 1F01209 T 5KCTC 32574 T ).
doi:10.1099/ijs.0.061234-0 pmid:24907265 fatcat:dp3jgb5xgneqppvtr4qhtheqxi