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Long-span, mate-pair scaffolding and other methods for faster next-generation sequencing library creation The NxSeq™ 40 kb Mate-Pair Cloning Kit facilitates the creation of scaffolds for de novo genome assembly. Supporting either Illumina or 454 sequencing, the kit produces long-span, mate-pair sequences with greater efficiency than existing protocols. In addition, NxSeq DNA Sample Prep Kits can be used to streamline workflow and speed up DNA library preparation for next-generation sequencing.doi:10.1038/nmeth.f.358 fatcat:bj3r3nx7are4vjo77f2hvgatiq