Long-span, mate-pair scaffolding and other methods for faster next-generation sequencing library creation

Cheng-Cang Wu, Rosa Ye, Svetlana Jasinovica, Megan Wagner, Ronald Godiska, Amy Hin-Yan Tong, Si Lok, Amanda Krerowicz, Curtis Knox, David Mead, Michael Lodes
2012 Nature Methods  
Long-span, mate-pair scaffolding and other methods for faster next-generation sequencing library creation The NxSeq™ 40 kb Mate-Pair Cloning Kit facilitates the creation of scaffolds for de novo genome assembly. Supporting either Illumina or 454 sequencing, the kit produces long-span, mate-pair sequences with greater efficiency than existing protocols. In addition, NxSeq DNA Sample Prep Kits can be used to streamline workflow and speed up DNA library preparation for next-generation sequencing.
more » ... arge insert mate-pair reads have a major impact on the overall success of de novo genome assembly and the discovery of inherited and acquired structural variants. Molecular tools are required that bridge the gap between massively parallel short-read sequencing technologies (35-1,500 bases) and the large scaffolds (40 kb or larger) needed to accurately assemble complex repeat-rich genomes. The NxSeq™ 40 kb Mate-Pair Cloning Kit has been developed to facilitate genome assembly and gap closure. Results show that approximately 40 kilobases (kb) paired-end sequences can be obtained by either
doi:10.1038/nmeth.f.358 fatcat:bj3r3nx7are4vjo77f2hvgatiq