Elongation-Competent Pauses Govern the Fidelity of a Viral RNA-Dependent RNA Polymerase

David Dulin, Igor D. Vilfan, Bojk A. Berghuis, Susanne Hage, Dennis H. Bamford, Minna M. Poranen, Martin Depken, Nynke H. Dekker
2015 Cell Reports  
Graphical Abstract Highlights d The RNAP elongation dynamic of dsRNA virus is captured by magnetic tweezers d Large sets of high-resolution data allow quantification of polymerase fidelity d Maximum likelihood estimation allows extraction of critical model parameters d Errors are predominantly incorporated via new error-prone catalytic pathway
doi:10.1016/j.celrep.2015.01.031 pmid:25683720 fatcat:vyo36nulofhehbxmlajcajqk6e