Non-hemic priming cardiopulmonary bypass (CPB) is available in the adult, but it is difficult in infants or children. The purpose of this investigation is to examine if it is possible to perform it in dogs by the aid of artificial blood i. e. perfluorochemical (PFC) emulsion. Seventy-five % of autologous blood, which is to be transfused after CPB, was removed in 16 dogs weighing around 10Kg and artificial blood CPB was performed. Nine dogs were used to determine the appropriate PFC

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... (conc.), O2/blood flow ratio, PO2, PCO2, and pH of blood, and to study CO2 elimination. Survival and complications after 45 to 50 minutes artificial blood CPB were evaluated with previously withdrawn autologous blood transfusion after the end of CPB. The hemoglobin conc. of blood during CPB was 1.3•}0.3Gm/dl. The O2 delivery was satisfactory when PFC conc. was 24Gm/dl, PaO2 over 500mmHg (O2/blood flow ratio 1 to 1.5), and perfusion rate over 120ml/KgB. W./min. The CO2 elimination was normal. The A-V pH difference was large aruund 0.3, due to a large A-V PCO2 difference, which was around 20mmHg. The PCO2 and pH therefore were regulated to maintain the normal values in venous blood rather than arterial blood in survival examination. Weaning from CPB was uneventful in all dogs.