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Colonization of Rice Roots by a Green Fluorescent Protein-Tagged Isolate of Ustilaginoidea virens
2015
American Journal of Plant Sciences
The fungus U. virens is the causal agent of rice false smut disease. The green fluorescent protein (GFP) was used to mark this fungus in order to visualize and analyze the colonization and infection processes in vivo. Using epifluorescence microscopy colonization and infection on rice roots were visualized in vivo. After inoculation for 2 to 15 d, it was observed that the conidia and their germ-tubes had penetrated into epidermis of young roots. The hyphae were found inside the root xylem 18 d
doi:10.4236/ajps.2015.614230
fatcat:ar4c7ulxs5davf2omcicsi7tlm