Enzymatic Saccharification of Laminaria japonica by Cellulase for the Production of Reducing Sugars

Eun Young Park, Jung Kyu Park
2020 Energies  
Enzymatic saccharification of Laminaria japonica seaweed biomass was optimized by four independent factors (enzyme dose, hydrolysis time, pH, and temperature) using response surface methodology (RSM). To confirm the significance of the quadratic model, an analysis of variance (ANOVA) was performed, and the F-value of 8.76 showed that the regression model was highly significant (≤0.1%). In the accuracy study, average recoveries were in the range of 97.00% to 98.32%. The optimum experimental
more » ... tions were an enzyme dose of 8.2%, a hydrolysis time of 26 h, a pH of 4.1, and a temperature of 43 °C. Temperature was the most important factor in the enzymatic saccharification. A relatively low temperature and short hydrolysis time were shown to improve the yield of reducing sugars.
doi:10.3390/en13030763 fatcat:txo6i7tipnb77ck6uf6t63b7lu