3 The abbreviations used are: HDAC, histone deacetylase; HAT, histone acetyltransferase; HDI, HDAC inhibitor; TSA, trichostatin A; p53RE, p53 response element; ChIP, chromatin immunoprecipitation; POL II, polymerase II; TBP, TATA box-binding protein; CBP, CREB (cAMP-response element-binding protein)-binding protein. http://www.jbc.org/ Downloaded from FIGURE 4. Association of transcription factors with p21 promoter after treatment with CG-1521 or TSA. A, schematic of the p21 promoter showing

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... TATA box, Ϫ1.3 and Ϫ2.4 kb p53 response elements, and control region at Ϫ5 kb. B, protein extracts from untreated (Ϫ) or CG-1521-and TSA-treated (ϩ) LNCaP cells after 8 h of treatment were subjected to ChIP analysis of the proximal promoter using antibodies against p300, CBP, HDAC1, HDAC2. C, ChIP analysis of the basal promoter using antibodies against Pol II, TBP, and TFIIH. Control immunoprecipitations with normal IgGs were also performed (Mock).