Metabolite Profiling in Arabidopsis [chapter]

Oliver Fiehn
Arabidopsis Protocols  
Metabolite profiling is the multiparallel relative quantification of a mixture of compounds or 15 compound classes using chromatography and universal detection technologies (GC-MS, LC-MS). In this respect it is an extension of classical single target methods from which it can be distinguished by its broader view on profiling major biochemical events. This broader scope of analysis outweighs the disadvantages by taking compromises in method development and the reduced accuracy for specific
more » ... for specific metabolites. This chapter exemplifies the strategies in 20 metabolite profiling of polar compounds by GC-MS. It gives experimental details on the basic steps:
doi:10.1385/1-59745-003-0:439 pmid:16739598 fatcat:se5haz7tlfecbdehlxoi6yaoom