Workers in fish smoking industry continue to be exposed to high concentrations of PM2.5 that contain various free radicals. PM2.5 are known to pose great health risk because of their ability to penetrate deeply into lungs and bloodstream. This study aims to analyze the relationship between PM2.5 concentration, years of work and duration of work with MDA levels. The present study used cross-sectional design with 104 workers in fish smoking industry Bandarharjo Semarang as subjects. Data were

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... ected through measurements of PM2.5 concentrations in the air, blood sampling for MDA levels test, and questionnaires. The results showed that PM2.5 concentrations in small and medium scale smoking houses were higher than the large one. The average MDA levels was 0.996 µg/mL. There was a significant association between MDA levels with PM2.5 concentrations (p = 0.007) and years of work (p = 0.019), while the duration of work did not show any significant association (p = 0.616). Correlation test results showed that there were no variables that have a strong asscociation with MDA levels. These findings need to be followed up by increasing coaching for workers, improving air circulation, and regulating working hours of workers, so that health risks can be minimized.