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Bestimmung des Molekulargewichtes der 20-β-Hydroxy-steroid-dehydrogenase
1962
Zeitschrift für Naturforschung. B, A journal of chemical sciences
The enzyme 20-β-Hydroxy-steroid-dehydrogenase obtained from the culture of Streptomyces hydrogenans and dissolved in 0.05 M Tris puffer, pH 7.3, has been investigated by means of a ultracentrifuge at 20 °C. The sedimentation- as well as the diffusion-coefficients obtained from various solutions at different concentrations were extrapolated to the concentration c = 0. The resulting zero-value for the sedimentation coefficient is s0 = 6.64 s and for the diffusion coefficient is D0 = 5.51 × 10-7
doi:10.1515/znb-1962-0704
fatcat:g7h565wen5dl3evngn6p4sv23i