Additional file 1: Table S1. Kinetic constants (Km and Vmax) of Pdc enzymes, Figure S1. Batch cultivation of the BD4 strain and the BD5 strain in minimal medium, Figure S2. Amino acid sequence of the C. tropicalis pyruvate decarboxylase I, Figure S3. In vitro Pdc activities in the control and four engineered S. cerevisiae strains expressing C. tropicalis pyruvate decarboxylase gene (CtPDC1) differentially, Figure S4. Fermentation profiles of the CtPDC1 expressing strains with glucose as a sole

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... arbon source in minimal medium, Figure S5. The NADH and NAD+ concentrations in the BD5_G1CtPDC1_nox strain with various aeration conditions, Figure S6. Profiles of batch cultivations for metabolomic analysis, Figure S7. Principal component analysis (PCA) of intracellular metabolite profiles of BD5_G1CtPDC1 and BD5_G1CtPDC1_nox in different aerobic conditions, Figure S8. Hierarchical clustering analysis (HCA) of metabolite profiles of BD5_G1CtPDC1 and BD5_G1CtPDC1_nox in different aerobic conditions, Figure S9. Comparison of intracellular metabolites between BD5_G1CtPDC1 at aerobic condition (Ct100) and BD5_G1CtPDC1_nox at microaerobic condition (N50), Figure S10. Specific glucose uptake rates of the wild type D452-2 and engineered strains.